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In this review we highlight the existence of circadian variations in ischemia and efficacy of thrombolytic intervention for clinicians, physicians, and pharmacists with the aim that time of day be recorded and reported as a biological variable in future clinical studies (Nelson and DeVries, 2017)

Washington, DC: Commissioned paper for the National Academies of Science, Engineering and Medicine NIA Decadal Study

After acclimation for 1 week, all rats were randomly divided into eight groups ( N = 5 per group), including CK group (normal feed (ND)), ABX group (ND + antibiotic cocktails (0.2 g/L metronidazole, 0.1 g/L vancomycin, 0.2 g/L ampicillin, and 0.2 g/L neomycin)), ABX + S-AM-0.6 group (antibiotic cocktails + 0.6 mg/kg of S-amlodipine + ND), ABX + S-AM-1.2 group, ABX + S-AM-5 group, S-AM-0.6 group (0.6 mg/kg of S-amlodipine + ND), S-AM-1.2 group (1.2 mg/kg of S-amlodipine + ND), and S-AM-5 group (5 mg/kg of S-amlodipine + ND), as shown in Figure 1

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