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Results:May vary from person to person

The reduction usually does not stop at the mixed-disulfide species because the second thiol of DTT has a high propensity to close the ring, forming oxidized DTT and leaving behind a reduced disulfide bond

Receiver operating characteristic curve analysis demonstrated the best diagnostic accuracy at an IGF-1 cutoff of1.493 SD, with 0.685 sensitivity, 0.417 specificity, 0.25 positive and 0.823 negative predictive values, and 0.517 area under the curve

Retained high 6xHis affinity in various buffers