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Community protocols and trial storage documentation describe avoiding freeze-thaw cycles on reconstituted vials freeze-thaw cycles are the documented cause of peptide structure damage

Elizarova S, Galstyan GR, Wolffenbuttel BHR (2014) Role of premixed insulin analogues in the treatment of patients with type 2 diabetes mellitus: a narrative review

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While these effects are typically temporary, lasting from a few days to weeks, it is crucial to consult your doctor or pharmacist if they persist, cause discomfort, or worsen in severity