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The DNA oxidation marker 8-hydroxy-2-deoxyguanosine (8-OHdG) is widely recognized as a surrogate indicator of ROS-mediated genotoxicity and has been associated with embryo loss and recurrent miscarriage in both natural conception and assisted reproductive technology (ART) settings (76, 83, 84)

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Briefly, cells were incubated with neutral red (70 mg l 1 in 0.1 ml DMEM-10, 37 C, 3060 min), washed 3X with PBS, re-suspended in lysis buffer (0.1 ml) and OD was measured with an ELISA reader at 540 nm

oleifera 150&250 mg/kg) showed significantly an increase in in the final body weight by 1.2fold and 1.34fold, respectively, as compared with CP group