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3 Results 3.1 Macrophage infection by HIV via cell-to-cell contact depends on viral tropism, macrophage polarization, and impairs osteoclast differentiation To investigate the determinants of HIV transmission from infected CD4+ T lymphocytes to macrophages (Mf), PBMC-derived Mf were first polarized into M0, M1, or M2 phenotypes and then co-cultured with Jurkat (Jk) cells: mock-infected (Jk-Mock), infected with R5- (Jk-R5) or X4-tropic (Jk-X4) HIV strains (see experimental timeline in Supplementary Figure S1A ), harboring comparable infection rate (25.5 2.7% and 24.0 6.4%, respectively

Rosa C, Franca C, Lanes S, Carvalho A, Penna A, Nogueira C, Luna M, Ramalho A

( Escherichia coli and purified with Ni 2+ affinity chromatography

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Importantly, miRNAs can be transported between cancer cells and stromal cells through extracellular vesicles known to mediate cell-to-cell communication in the tumor microenvironment ( in vitro and angiogenesis and metastasis of breast cancer in vivo (Zhou et al., 2014)